1-(2-Aminoethyl)-1H-pyrazol-4-ylphosphonic acid-linker/OVA is a versatile dye. These dyes are essential tools in biological experiments, aiding researchers in observing and analyzing cellular structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Their applications range from fundamental scientific research to clinical diagnostics. Additionally, dyes have extensive use in traditional fields like textile dyeing and emerging areas such as functional textile processing, food colorants, and dye-sensitized solar cells.