Name: EDC hydrochloride
Brand: TargetMol
SKU: T19947
Price: 167 CNY
Availability: InStock
Rating: 5 (10 reviews)

EDC hydrochloride

很棒

产品编号 T19947Cas号 25952-53-8

别名 EDC-HCl Crosslinker, 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

EDC hydrochloride (EDC-HCl Crosslinker) 是一种水溶性的碳二亚胺类化合物。EDC hydrochloride 作为缩合剂，通过羧基和氨基的缩合反应形成肽键，可用于合成具有特定序列和功能的肽。EDC hydrochloride 可将半抗原共价结合到载体蛋白上，形成免疫抗原，用于疫苗研究。EDC hydrochloride 能够与核酸的5′磷酸基团反应，用于 DNA 和 RNA 的标记。EDC hydrochloride 可将蛋白质与核酸交联，用于制备免疫交联物。EDC hydrochloride 作为交联剂，连接生物分子的胺反应性 NHS 酯与羧基，可用于蛋白质偶联，创造具有新特性和功能的杂交分子。

规格

价格

库存

数量

5 g

¥ 167

In stock

10 g

¥ 265

In stock

产品介绍

生物活性

产品描述	EDC hydrochloride (EDC-HCl Crosslinker) is a water-soluble carbodiimide compound. EDC hydrochloride, as a condensing agent, forms peptide bonds through the condensation reaction of carboxyl and amino groups and can be used to synthesize peptides with specific sequences and functions. EDC hydrochloride can covalently bind haptens to carrier proteins to form immune antigens, which are used in vaccine research. EDC hydrochloride can react with the 5 'phosphate group of nucleic acids and is used for the labeling of DNA and RNA. EDC hydrochloride can cross-link proteins with nucleic acids and is used to prepare immune cross-links. EDC hydrochloride, as a crosslinking agent, connects the amine-reactive NHS ester of biomolecules with the carboxyl group, which can be used for protein coupling to create hybrid molecules with new properties and functions.
靶点活性	lysine:500 µM
体外活性	方法：人成纤维细胞用EDC hydrochloride（4、 8、 16、 24 mM）处理4、 6、24小时，检测显示细胞存活率。结果：细胞存活率随EDC hydrochloride浓度和时间依赖性方式降低。
体内活性	方法：为研究EDC hydrochloride对神经功能的影响，将EDC hydrochloride（0.0075%，一次）脊髓注射给成年Swiss Webster 雄性小鼠。结果：EDC hydrochloride不会出现注射后神经功能缺损。[1]
别名	EDC-HCl Crosslinker, 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

化学信息

分子量	191.7
分子式	C₈H₁₈ClN₃
CAS No.	25952-53-8
Smiles	Cl.CCN=C=NCCCN(C)C
密度	0.877 g/cm3 at 20℃ (lit.)
颜色	White
物理性状	Solid

储存&溶解度

存储

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度信息

H2O: 30 mg/mL (156.49 mM)

DMSO: 92 mg/mL (479.92 mM), Sonication is recommended.

溶液配制表

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	5.2165 mL	26.0824 mL	52.1648 mL	260.8242 mL
5 mM	1.0433 mL	5.2165 mL	10.4330 mL	52.1648 mL
10 mM	0.5216 mL	2.6082 mL	5.2165 mL	26.0824 mL
20 mM	0.2608 mL	1.3041 mL	2.6082 mL	13.0412 mL
50 mM	0.1043 mL	0.5216 mL	1.0433 mL	5.2165 mL
100 mM	0.0522 mL	0.2608 mL	0.5216 mL	2.6082 mL

体内实验配液计算器