SIRP alpha V4 Protein, Human, Recombinant (His & Avi)

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

规格	价格/CNY		数量
100 μg	￥ 2,660	5日内发货
500 μg	￥ 10,600	5日内发货
1 mg	￥ 17,800	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 2,660

5日内发货

500 μg

￥ 10,600

5日内发货

1 mg

￥ 17,800

5日内发货

生物活性

Immobilized Human SIRP alpha V4, His Tag at 1μg/ml (100μl/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA.

产品描述

种属	Human
表达系统	HEK293
标签	C-His-Avi
蛋白编号	P78324 variant 4
别名	BIT, SIRP α V4, MFR, SHPS1, SIRPA, SIRP α, P84, MYD1, MYD-1, PTPNS1, SIRP alpha, SHPS-1, CD172a
蛋白构建	Recombinant Human SIRP alpha V4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Arg370(E33G, L44S, T50S, T52I, R54H).[Accession \|P78324 variant 4]
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

内毒素	Less than 1EU per μg by the LAL method.
缓冲液	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.