DC-LC3in-D5 is a potent and selective covalent inhibitor of LC3A/B that disrupts autophagy by covalently binding to Lys49 on LC3B (IC₅₀ = 200 nM), resulting in impaired LC3B lipidation, accumulation of the autophagy substrate p62, and a significant reduction in the formation of autophagosome vesicles.

DC-LC3in-D5 在 HeLa 细胞中具有高度选择性地靶向 LC3A/B 的能力。体外实验显示，DC-LC3in-D5（3–30 μM，处理 16 小时）可阻断 LC3B 的脂质化过程、抑制自噬泡形成，并导致 p62 显著积累，提示其可有效干扰自噬过程[1]。