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顶部生物活性储存和溶解信息计算器

Anti-RAF1 Antibody (1T539)

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产品编号 TMAC-03521

Anti-RAF1 Antibody (1T539) 是一种 Rabbit 抗体，靶向 RAF1。Anti-RAF1 Antibody (1T539) 可用于 ICC/IF,WB。

Anti-RAF1 Antibody (1T539)

Anti-RAF1 Antibody (1T539)

一键复制产品信息
Rating icon 还可以
产品编号 TMAC-03521

Anti-RAF1 Antibody (1T539) 是一种 Rabbit 抗体，靶向 RAF1。Anti-RAF1 Antibody (1T539) 可用于 ICC/IF,WB。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-RAF1 Antibody (1T539) is a Rabbit antibody targeting RAF1. Anti-RAF1 Antibody (1T539) can be used in ICC/IF,WB.
Ig Type
IgG
克隆号
1T539
交叉反应
Human
验证活性
1. Western blot analysis of Raf1 on HepG2 cells lysates using anti-Raf1 antibody at 1/1,000 dilution.
2. ICC staining Raf1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Raf1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivity
应用ICC/IFWB
推荐剂量
WB: 1:1000-2000; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P04049
化学信息
分子量Theoretical: 73 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

Raf-1RAF1RAF proto-oncogene serine/threonine-protein kinaseRAF 1RAFProto-oncogene c-RAF (cRaf)c-RafcRaf

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4个月前
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