Anti-MAPK3/1 Antibody (1G244) is a Rabbit antibody targeting MAPK3/1. Anti-MAPK3/1 Antibody (1G244) can be used in WB,ICC/IF,IP,FCM.

IgG

Human,Mouse,Rat,zebrafish

1. Western blot analysis of ERK1/2 on different cell lysates using anti-ERK1/2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: SW480, Lane 3: HCT116, Lane 4: PC12.
2. Western blot analysis of ERK1/2 on hybrid fish (crucian-carp) brain tissue lysate using anti-ERK1/2 antibody at 1/500 dilution.
3. ICC staining ERK1/2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining ERK1/2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining ERK1/2 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining ERK1/2 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Hela cells with ERK1/2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
8. Flow cytometric analysis of SH-SY-5Y cells with ERK1/2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

WB: 1:1000-5000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

Unconjugated

Recombinant Protein