Anti-CHGA Antibody (8R99)

Name: Anti-CHGA Antibody (8R99)
Brand: TargetMol
SKU: TMAY-00103
Rating: 4.5 (1 reviews)

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产品编号 TMAY-00103

Anti-CHGA Antibody (8R99) 是一种 Rabbit 抗体，靶向 CHGA。Anti-CHGA Antibody (8R99) 可用于 WB,ICC/IF。

Anti-CHGA Antibody (8R99)

还可以

产品编号 TMAY-00103

Anti-CHGA Antibody (8R99) 是一种 Rabbit 抗体，靶向 CHGA。Anti-CHGA Antibody (8R99) 可用于 WB,ICC/IF。

规格	价格	库存	数量
100 μL	¥ 2,294	5日内发货

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生物活性

产品描述	Anti-CHGA Antibody (8R99) is a Rabbit antibody targeting CHGA. Anti-CHGA Antibody (8R99) can be used in WB,ICC/IF.
Ig Type	Monoclonal Rabbit IgG
克隆号	8R99
交叉反应	Human
验证活性	1. Anti-ChromograninA rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: SH-SY5Y Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:50 kDa. -Observed band size:50 kDa. 2. Immunofluorescence staining of CHGA in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CHGA monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm .
应用	WB,ICC/IF
推荐剂量	WB: 1:500-1:1000; ICC-IF: 1:20-1:100
抗体种类	Monoclonal
宿主来源	Rabbit
构建方式	This antibody was obtained from a rabbit immunized with A synthetic peptide corresponding to the N-terminus of the human Chromogranin A/CHGA/CGA
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	The acidic glycoprotein chromogranin A (CHGA) is co-stored/co-secreted with catecholamines and crucial for secretory vesicle biogenesis in neuronal/neuroendocrine cells. CHGA is dysregulated in several cardiovascular diseases, but the underlying mechanisms are not well established. The CHGA promoter haplotype 2 (occurring in a large proportion of the world population) in enhancing CHGA expression in haplotype 2 carriers who may be at higher risk for cardiovascular/metabolic disorders. Chromogranin A (CHGA) plays a fundamental role in the biogenesis of catecholamine secretory granules. Changes in storage and release of CHGA in clinical and experimental hypertension prompted us to study whether genetic variation at the CHGA locus might contribute to alterations in autonomic function, and hence hypertension and its target organ consequences such as hypertensive renal disease (nephrosclerosis). Chromogranin A (CHGA) plays a catalytic role in formation of catecholamine storage vesicles and also serves as precursor to the peptide fragment catestatin, a catecholamine secretory inhibitor whose expression is diminished in the hypertensive individuals. The chromogranin/secretogranin proteins are costored and coreleased with catecholamines from secretory vesicles in chromaffin cells and noradrenergic neurons. Chromogranin A (CHGA) regulates catecholamine storage and release through intracellular (vesiculogenic) and extracellular (catecholamine release-inhibitory) mechanisms. CHGA is a candidate gene for autonomic dysfunction syndromes, including intermediate phenotypes that contribute to human hypertension.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	A synthetic peptide corresponding to the N-terminus of the human Chromogranin A/CHGA/CGA
抗原种属	Human

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.