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Anti-CHGA Antibody (5H899)
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产品编号 TMAC-00856
Anti-CHGA Antibody (5H899) 是一种 Mouse 抗体，靶向 CHGA。Anti-CHGA Antibody (5H899) 可用于 WB,IHC,ICC。
Anti-CHGA Antibody (5H899)
Anti-CHGA Antibody (5H899)
还可以产品编号 TMAC-00856
Anti-CHGA Antibody (5H899) 是一种 Mouse 抗体，靶向 CHGA。Anti-CHGA Antibody (5H899) 可用于 WB,IHC,ICC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,985 | 5日内发货 | |
| 100 μL | ¥ 2,990 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-CHGA Antibody (5H899) is a Mouse antibody targeting CHGA. Anti-CHGA Antibody (5H899) can be used in WB,IHC,ICC. |
| 克隆号 | 5H899 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Chromogranin A on PC-12 using anti-Chromogranin A antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin. 5. ICC staining Chromogranin A(red) in Hela cells. The nuclear counter stain is DAPI(blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Chromogranin A(red) in LOVO cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Chromogranin A(red) in PANC-1 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:500; IHC: 1:50-200; ICC: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granules of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A (Chr-A), chromogranin B (Chr-B, also known as secretogranin I) chromogranin C (also known as secretogranin II or Sg II), secretogranin III (Sg III or SCG3). High levels of Chr-A expression is a characteristic of neuroendocrine tumors. Pancreastatin is a peptide derived from Chr-A which inhibits insulin secretion, exocrine pancreatic secretion and gastric acid secretion. Pancreastatin exists as two forms; the major form is expressed in stomach and colon extracts. In neuroendocrine cells the level Sg II has been shown to increase four-fold in response to histamine, while levels of Chr-A and Chr-B showed little or no increase. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes. |
| 偶联 | Unconjugated |
| 免疫原 | Peptide |
| Uniprot ID |
| 分子量 | Theoretical: 86 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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