store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PAF (C16) 是有效的 MAPK 和 MEK/ERK 激活剂,可诱导血管通透性增加。PAF (C16) (PAF (C16)) 是血小板活化因子,是一种磷脂衍生介质,也是 PAF G 蛋白偶联受体 (PAFR) 的配体。PAF (C16) 在体外实验中显示出抗凋亡和抗炎活性,通过与其受体 (PAF-R) 相互作用以执行细胞信号传导来抑制 caspase 依赖性凋亡。
产品描述 | PAF (C16) is a potent MAPK and MEK/ERK activator that induces increased vascular permeability. PAF (C16) (PAF (C16)) is a platelet-activating factor, a phospholipid-derived mediator and a ligand for PAF G protein-coupled receptor (PAFR). PAF (C16) has shown anti-apoptotic and anti-inflammatory activity in vitro, inhibiting Caspase-dependent apoptosis by interacting with its receptor (PAF-R) to perform cell signaling. |
体外活性 |
In PAFR+/+ cultures, C16-PAF (PAF (C16); 0.5-1.5 μM; for 24 hours) does not elicit significant concentration-dependent neuronal loss, whereas it does induce significant concentration-dependent neuronal loss in PAFR?/? cultures. Specifically, C16-PAF (1 μM) induces neuronal death in PAFR?/? cells infected with EGFP alone[1].In PAFR neurons, C16-PAF (1 μM; for 24 hours) activates caspase 7 but not caspase 3[1]. Synthesized by two distinct pathways, the remodeling pathway and the de novo synthesis pathway, C16-PAF acts by binding to a unique G-protein-coupled seven-transmembrane receptor[2][3]. Furthermore, C16-PAF (1-25 μg/ml; 6, 12, 24 h) exhibits time-dependent inhibition of M. smegmatis and M. bovis BCG growth[3]. |
别名 | C16-PAF |
分子量 | 523.68 |
分子式 | C26H54NO7P |
CAS No. | 74389-68-7 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 30 mg/mL (57.29 mM), Sonication is recommended.
DMSO: 40 mg/mL (76.38 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO | 1 mM | 1.9096 mL | 9.5478 mL | 19.0956 mL | 47.7391 mL |
5 mM | 0.3819 mL | 1.9096 mL | 3.8191 mL | 9.5478 mL | |
10 mM | 0.191 mL | 0.9548 mL | 1.9096 mL | 4.7739 mL | |
20 mM | 0.0955 mL | 0.4774 mL | 0.9548 mL | 2.387 mL | |
50 mM | 0.0382 mL | 0.191 mL | 0.3819 mL | 0.9548 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
PAF (C16) 74389-68-7 MAPK Metabolism ERK MEK Endogenous Metabolite C16-PAF C16 PAF C-16 C 16 Inhibitor inhibitor inhibit