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Anti-ERK1/2 Antibody (4U909)
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产品编号 TMAB-00636
别名 Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1
Anti-ERK1/2 Antibody (4U909) 是一种 Mouse 抗体，靶向 ERK1/2。Anti-ERK1/2 Antibody (4U909) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-ERK1/2 Antibody (4U909)
Anti-ERK1/2 Antibody (4U909)
还可以产品编号 TMAB-00636 别名 Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1
Anti-ERK1/2 Antibody (4U909) 是一种 Mouse 抗体，靶向 ERK1/2。Anti-ERK1/2 Antibody (4U909) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-ERK1/2 Antibody (4U909) is a Mouse antibody targeting ERK1/2. Anti-ERK1/2 Antibody (4U909) can be used in WB,IHC-P,IHC-Fr,IF. |
| 别名 | Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1 |
| Ig Type | IgG |
| 克隆号 | 4U909 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00636) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00636) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00636) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 4. 25 μg total protein per Lane of various lysates probed with ERK1/2 monoclonal antibody, unconjugated (TMAB-00636) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 5. 25 μg total protein per Lane of various lysates probed with ERK1/2 monoclonal antibody, unconjugated (TMAB-00636) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Note=Associated with the spindle duringprometaphase and metaphase. PEA15-binding andphosphorylated DAPK1 promote its cytoplasmic retention.Phosphorylation at Ser-244 and Ser-246 as well asautophosphorylation at Thr-188 promote nuclear localization. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008]. |
| 免疫原 | KLH conjugated synthetic peptide: human ERK1/2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Tangles & Tau,MAPK Pathway,Cytoplasmic |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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