Anti-p53 Antibody (2H232)

Name: Anti-p53 Antibody (2H232)
Brand: TargetMol
SKU: TMAH-01202
Rating: 4.5 (1 reviews)

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产品编号 TMAH-01202

Anti-p53 Antibody (2H232) 是一种抗体，靶向 p53。Anti-p53 Antibody (2H232) 可用于 ELISA, IHC。

Anti-p53 Antibody (2H232)

还可以

产品编号 TMAH-01202

Anti-p53 Antibody (2H232) 是一种抗体，靶向 p53。Anti-p53 Antibody (2H232) 可用于 ELISA, IHC。

规格	价格	库存	数量
50 μL	¥ 1,315	5日内发货
100 μL	¥ 2,190	5日内发货

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生物活性

产品描述	Anti-p53 Antibody (2H232) is an antibody targeting p53. Anti-p53 Antibody (2H232) can be used in ELISA, IHC.
Ig Type	Rabbit IgG
克隆号	2H232
交叉反应	Human
验证活性	IHC image of TMAH-01202 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用	ELISA, IHC
推荐剂量	IHC:1:50-1:200.
抗体种类	Monoclonal
亚细胞定位	Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.; [Isoform 1]: Nucleus. Cytoplasm. Note=Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4.; [Isoform 2]: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 3]: Nucleus. Cytoplasm. Note=Localized in the nucleus in most cells but found in the cytoplasm in some cells.; [Isoform 4]: Nucleus. Cytoplasm. Note=Predominantly nuclear but translocates to the cytoplasm following cell stress.; [Isoform 7]: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 8]: Nucleus. Cytoplasm. Note=Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli.; [Isoform 9]: Cytoplasm.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景	Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	A synthetic peptide: Human p53 (acetyl K370)
抗原种属	Human
基因ID	7157
Uniprot ID	P04637
研究领域	Epigenetics and Nuclear Signaling, Cancer, Cell biology