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Anti-JUN Antibody (1D644) 是一种抗体,靶向 JUN。Anti-JUN Antibody (1D644) 可用于 ELISA,IHC,WB。
Anti-JUN Antibody (1D644) 是一种抗体,靶向 JUN。Anti-JUN Antibody (1D644) 可用于 ELISA,IHC,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
| 产品描述 | Anti-JUN Antibody (1D644) is an antibody targeting JUN. Anti-JUN Antibody (1D644) can be used in ELISA,IHC,WB. |
| Ig Type | Rabbit IgG |
| 克隆号 | 1D644 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: 293 whole cell lysate -All lanes: JUN antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 36 kDa -Observed band size: 43 kDa 2. IHC image of TMAH-00657 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISAIHCWB |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in the developing and adult prostate and prostate cancer cells. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human c-Jun |
| 抗原种属 | Human |
| 基因名称 | JUN |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cancer, Immunology, Signal transduction |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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