Powder: -20°C for 3 years | In solvent: -80°C for 1 year
STO-609 是一种特异性和细胞渗透性的 Ca2+/钙调蛋白依赖性蛋白激酶激酶抑制剂,用于重组 CaM-KKα 和 CaM-KKβ,Ki 值分别为 80 和 15 ng/mL。它抑制 Hela 细胞溶解物中的 AMP 活化蛋白激酶激酶活性,IC50值约为 0.02 g/ml。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 333 | 现货 | ||
5 mg | ¥ 795 | 现货 | ||
10 mg | ¥ 1,280 | 现货 | ||
25 mg | ¥ 2,680 | 现货 | ||
50 mg | ¥ 3,950 | 现货 | ||
100 mg | ¥ 5,630 | 现货 | ||
500 mg | ¥ 11,300 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 935 | 现货 |
产品描述 | STO-609 is a specific and cell-permeable inhibitor of the Ca2+/calmodulin-dependent protein kinase kinase (CaM-KK) for recombinant CaM-KKα/KKβ (Ki: 80/15 ng/mL). |
靶点活性 | CaM-KKα:0.25 μM(Ki), CaM-KKβ:47 nM(Ki) |
体外活性 | STO-609 also inhibits their autophosphorylation activities. STO-609 is highly selective for CaM-KK without any significant effect on the downstream CaM kinases (CaM-KI and -IV), and the IC50 value of the compound against CaM-KII is 10 μg/mL. STO-609 inhibits constitutively active CaM-KKα as well as the wild-type enzyme. In transfected HeLa cells, STO-609 dose-dependently suppresses the Ca2+-induced activation of CaM-KIV. STO-609 (1μg/mL) significantly reduces the endogenous activity of CaM-KK in SH-SY5Y neuroblastoma cells (80%). |
别名 | STO 609 |
分子量 | 314.29 |
分子式 | C19H10N2O3 |
CAS No. | 52029-86-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 4.17 mg/mL (13.26 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.1818 mL | 15.9089 mL | 31.8177 mL | 79.5444 mL |
5 mM | 0.6364 mL | 3.1818 mL | 6.3635 mL | 15.9089 mL | |
10 mM | 0.3182 mL | 1.5909 mL | 3.1818 mL | 7.9544 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
STO-609 52029-86-4 Autophagy Chromatin/Epigenetic Neuroscience PI3K/Akt/mTOR signaling CaMK AMPK AMP-activated protein kinase STO609 Calmodulin-dependent protein kinases STO 609 Inhibitor Calmodulin-dependent kinases inhibit inhibitor