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Anti-AMPK alpha 1 Antibody (4D648)
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产品编号 TMAB-00113
Anti-AMPK alpha 1 Antibody (4D648) 是一种 Mouse 抗体，靶向 AMPK alpha 1。Anti-AMPK alpha 1 Antibody (4D648) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-AMPK alpha 1 Antibody (4D648)
Anti-AMPK alpha 1 Antibody (4D648)
还可以产品编号 TMAB-00113
Anti-AMPK alpha 1 Antibody (4D648) 是一种 Mouse 抗体，靶向 AMPK alpha 1。Anti-AMPK alpha 1 Antibody (4D648) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-AMPK alpha 1 Antibody (4D648) is a Mouse antibody targeting AMPK alpha 1. Anti-AMPK alpha 1 Antibody (4D648) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG1 |
| 克隆号 | 4D648 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Sample: HL60 (Human) Cell Lysate at 30 μg Primary: Anti-AMPK alpha-1 (TMAB-00113) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha-1) Monoclonal Antibody, Unconjugated (TMAB-00113) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha-1) Monoclonal Antibody, Unconjugated (TMAB-00113) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:200-800; IHC-Fr: 1:200-500; IF: 1:200-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| 免疫原 | KLH conjugated synthetic peptide: human AMPK alpha-1 |
| 抗原种属 | Human |
| 基因ID | |
| 研究领域 | Integration of energy metabolism,Response to hypoxia,Metabolism,Integration of energy,Fatty acids,Hypoxia,Fatty acid oxidation,Cancer,Neurodegenerative disease,Amyloid,Other Kinases |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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