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AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST)

产品编号 TMPY-04454

AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&O43741&P54646.

AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST)

产品编号 TMPY-04454

规格	价格	库存	数量
50 μg	¥ 5,750	5日内发货

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产品信息

生物活性	The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
产品描述	AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&O43741&P54646.
种属	Human
表达系统	Baculovirus Insect Cells
标签	His, GST
蛋白编号	P54619&O43741&P54646
别名	AMPK, AMPK(α2, β1, γ1)
蛋白构建	A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. Predicted N terminal: Met & Met & His
蛋白纯度	> 94 % as determined by SDS-PAGE
分子量	158 kDa (predicted); 40, 42 & 95 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10% gly, 1 mM GSH, pH 7.4.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.