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AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&O43741&P54646.
别名 AMPK, AMPK(α2, β1, γ1)
AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&O43741&P54646.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 987 | 6-8日内发货 | |
| 10 μg | ¥ 1,720 | 6-8日内发货 | |
| 20 μg | ¥ 2,890 | 5日内发货 | |
| 50 μg | ¥ 6,670 | 5日内发货 |
| 产品描述 | AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&O43741&P54646. |
| 生物活性 | The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
| 种属 | Human |
| 表达系统 | Baculovirus Insect Cells |
| 标签 | His, GST |
| 蛋白编号 | P54619&O43741&P54646 |
| 蛋白构建 | A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. Predicted N terminal: Met & Met & His |
| 蛋白纯度 | > 94 % as determined by SDS-PAGE |
| 蛋白性状 | Solution |
| 缓冲液 | Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10% gly, 1 mM GSH, pH 7.4. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 别名 | AMPK, AMPK(α2, β1, γ1) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 分子量 | 158 kDa (predicted); 40, 42 & 95 kDa (reducing conditions) |
| 运输方式 | Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
| 存储 |
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