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AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST)

Synonyms: AMPK, AMPK(α2, β2, γ1)
货号 TMPY-04453 一键复制产品信息
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AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&Q9Y478&P54646.

AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST)

一键复制产品信息
Rating icon 还可以
货号 TMPY-04453

别名 AMPK, AMPK(α2, β2, γ1)

AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&Q9Y478&P54646.

AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST)
规格价格库存数量
5 μg
¥ 987
6-8日内发货
10 μg
¥ 1,720
6-8日内发货
20 μg
¥ 2,890
5日内发货
50 μg
¥ 6,670
5日内发货
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产品介绍


生物活性
产品描述
AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&Q9Y478&P54646.
生物活性
The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
种属
Human
表达系统
Baculovirus Insect Cells
标签His, GST
蛋白编号P54619&Q9Y478&P54646
蛋白构建A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. Predicted N terminal: Met & Met & His
蛋白纯度
> 90 % as determined by SDS-PAGE
蛋白性状Solution
缓冲液Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10% gly, 1 mM GSH, pH 7.4.
复溶方法A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
别名AMPK, AMPK(α2, β2, γ1)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量158 kDa (predicted); 35, 37 & 95 kDa (reducing conditions)
储存&溶解度
运输方式Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
存储

计算器

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剂量转换

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技术支持

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