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AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Synonyms: AMPK, AMPK(α1, β1, γ1)
货号 TMPY-04451 一键复制产品信息 纯度: 95.5%
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AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

一键复制产品信息
Rating icon 很棒

纯度: 95.5%

货号 TMPY-04451

别名 AMPK, AMPK(α1, β1, γ1)

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)
规格价格库存数量
5 μg
¥ 987
现货
10 μg
¥ 1,690
现货
20 μg
¥ 2,880
3日内发货
50 μg
¥ 5,770
现货
100 μg
¥ 9,830
5日内发货
200 μg
¥ 16,770
5日内发货
500 μg
¥ 33,870
5日内发货
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纯度: 95.5%
颜色: 无色透明
性状: Solution
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产品介绍


生物活性
产品描述
AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.
生物活性
The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
种属
Human
表达系统
Baculovirus Insect Cells
标签His, GST
蛋白编号P54619-1&Q9Y478-1&Q13131-1
蛋白构建A DNA sequence encoding the human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie. Predicted N terminal: Met & Met & Met
蛋白纯度
> 85 % as determined by SDS-PAGE
AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)
蛋白性状Solution
缓冲液Supplied as sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5.
复溶方法A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
别名AMPK, AMPK(α1, β1, γ1)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量160 kDa (predicted); 38 (PRKAB1), 40 (PRKAG1) & 95 (PRKAA1) kDa (reducing conditions)
储存&溶解度
运输方式Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
存储

计算器

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剂量转换

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技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.
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