AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells

规格	价格/CNY		数量
50 μg	￥ 5,770	现货
100 μg	￥ 9,840	5日内发货
200 μg	￥ 16,770	5日内发货
500 μg	￥ 33,870	5日内发货

规格

价格/CNY

货期

数量

50 μg

￥ 5,770

现货

100 μg

￥ 9,840

5日内发货

200 μg

￥ 16,770

5日内发货

500 μg

￥ 33,870

5日内发货

生物活性	The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
产品描述	AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells

种属	Human
表达系统	Baculovirus-Insect Cells
标签	His,GST
蛋白编号	P54619-1&Q9Y478-1&Q13131-1
别名	AMPK, AMPK(α1, β1, γ1)
蛋白构建	A DNA sequence encoding the human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie.
蛋白纯度	> 85 % as determined by SDS-PAGE
分子量	Approxiamtely 160 kDa

内毒素	< 1.0 EU per μg of the protein as determined by the LAL method.
缓冲液	Supplied as sterile 20mM Tris, 300mM NaCl, 10% Glycerol, pH 7.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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