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AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μg | ¥ 5,770 | 现货 | |
100 μg | ¥ 9,840 | 5日内发货 | |
200 μg | ¥ 16,770 | 5日内发货 | |
500 μg | ¥ 33,870 | 5日内发货 |
生物活性 | The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
产品描述 | AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1. |
种属 | Human |
表达系统 | Baculovirus Insect Cells |
标签 | His, GST |
蛋白编号 | P54619-1&Q9Y478-1&Q13131-1 |
别名 | AMPK, AMPK(α1, β1, γ1) |
蛋白构建 | A DNA sequence encoding the human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie. Predicted N terminal: Met & Met & Met |
蛋白纯度 | > 85 % as determined by SDS-PAGE ![]() |
分子量 | 160 kDa (predicted); 38 (PRKAB1), 40 (PRKAG1) & 95 (PRKAA1) kDa (reducing conditions) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |