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TL1A/TNFSF15 Trimer Protein, Human, Recombinant (His & Flag)
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货号 TMPK-00143
别名 VEGI-251, VEGI192A, VEGI, TNFSF15, TL1A, TL1
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. TNFSF15 Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 58.2 kDa and the accession number is O95150-1.
其他形式的 “TL1A/TNFSF15 Trimer Protein, Human, Recombinant (His & Flag)”:
TL1A/TNFSF15 Trimer Protein, Human, Recombinant (His & Flag)
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货号 TMPK-00143 别名 VEGI-251, VEGI192A, VEGI, TNFSF15, TL1A, TL1
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. TNFSF15 Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 58.2 kDa and the accession number is O95150-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 343 | 现货 | |
| 10 μg | ¥ 549 | 现货 | |
| 20 μg | ¥ 898 | 现货 | |
| 50 μg | ¥ 1,760 | 3日内发货 | |
| 100 μg | ¥ 2,960 | 现货 | |
| 200 μg | ¥ 5,350 | 5日内发货 | |
| 500 μg | ¥ 11,800 | 5日内发货 |
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纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
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| 产品描述 | TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. TNFSF15 Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 58.2 kDa and the accession number is O95150-1. |
| 生物活性 | 1. Immobilized Human TNFSF15 Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA.
2. Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay. |
| 研究背景 | TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | N-His-Flag |
| 蛋白编号 | O95150-1 |
| 蛋白构建 | Asp91-Leu251 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 别名 | VEGI-251, VEGI192A, VEGI, TNFSF15, TL1A, TL1 |
| 内毒素 | < 1 EU/μg by the LAL method. |
| 分子量 | 58.2 kDa (predicted). Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result. |
| 颜色 | White |
| 物理性状 | Lyophilized powder |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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