Nanchangmycin (Nanchangmycin A), a polyether antibiotic, is similar to the structure of dianemycin. It is very potent against the broad spectrum of harmful nematodes and insects but not for mammals and plants.

Histone deacetylase activity:For PCI-34051 characterization, measurements are performed in a reaction volume of 100 μL using 96-well assay plates in a fluorescence plate reader. For each isozyme. The HDAC protein in reaction buffer (50 mM HEPES, 100 mM KCl, 0.001% Tween-20, 5% dimethyl sulfoxide, pH7.4, supplemented with bovine serum albumin at concentrations of 0-0.05%) is mixed with PCI-34051 at various concentrations and allowed to incubate for 15 min. Trypsin is added to a final concentration of 50 nM, and acetyl-Gly-Ala-(N-acetyl-Lys)-amino-4-methyl coumarin is added to a final concentration of 25-100 μM to initiate the reaction. After a 30 min lag time, the fluorescence is measured over a 30 min time frame using an excitation wavelength of 335 nm and a detection wavelength of 460 nm. The increase in fluorescence with time is used as the measure of the reaction rate.

5×10E3 U2OS cells were plated in 25ul media per well in 384-well plates and cultured for 24 hours. Add 50 nl of Nanchangmycin, which is 0.7uM dissolved in 0.2% DMSO, to each well incubating for 1 hour.

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 88.9 mg/mL (99.99 mM), Sonication is recommended.

10% DMSO+90% Corn Oil: 3.3 mg/mL (3.71 mM), Sonication is recommeded.