Powder: -20°C for 3 years | In solvent: -80°C for 1 year
MOPIPP 是新型的吲哚基查尔酮,可以透过血脑屏障。MOPIPP 对胶质母瘤细胞的肿瘤进展有抑制作用。MOPIPP 诱导细胞空泡化并增加自噬体数量。MOPIPP 还会触发细胞巨泡式死亡,并且中断葡萄糖摄取和糖酵解代谢。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 10,600 | 6-8周 | ||
50 mg | ¥ 13,800 | 6-8周 | ||
100 mg | ¥ 17,500 | 6-8周 |
产品描述 | MOPIPP is a novel indolebased chalcone that can cross the blood-brain barrier. MOPIPP inhibits the tumor progression agaisnt glioblastoma cells. MOPIPP induces cellular vacuolization as well as increases autophagosomes numbers. MOPIPP also triggers methuosis and distrupts glucose uptake and glycolytic metabolism [1] [2] [3]. |
体外活性 | MOPIPP (10 μM; 48 h) induces cellular vacuolization but does not cause cell death in U251 glioblastoma cells, exhibiting characteristics of late endosomes [1] [2]. MOPIPP (10 μM; 24 h) results an increasing LC3 fluorescence associated with the number of autophagosomes and inhibits fusion of autophagosomes with lysosomes [1]. MOPIPP (10 μM; 24 h) increases the amounts of exosomal marker proteins in vesicle fractions recovered from 293T cells [2]. MOMIPP (10 μM; 24 h and 5 h, respectively) causes early disruptions of glucose uptake and glycolytic metabolism, induces methuosis, a form of non-apoptotic cell death, in glioblastoma and other cancer cell lines [3]. MOMIPP (10 μM; 4 h and 24 h) selectively activates the JNK1/2 stress kinase pathway, resulting in phosphorylation of c-Jun, Bcl-2 and Bcl-xL [3]. Immunofluorescence [1] Cell Line: U251 glioblastoma cells Concentration: 10 μM Incubation Time: 24 hours; incubated with 2.5 μg/ml Acridine Orange for 45 min Result: Caused accumulation of autophagosome markers. Increased punctate LC3 fluorescence and suggested an increase in the number of autophagosomes in cells. Western Blot Analysis [3] Cell Line: U251 glioblastoma cells Concentration: 10 μM Incubation Time: 4 and 24 hours Result: Triggered increaseing phosphorylation of Bcl-2 and Bcl-xL, accompanied the activation of JNK. |
体内活性 | MOMIPP (80 mg/kg; i.p.; single dose) readily penetrates the bloodbrain barrier in female Swiss Webster Mice and (80 mg/kg; i.p.; every 24 h; 15 d) is effective in suppressing progression of intracerebral glioblastoma xenografts in female NCR-Foxn1 mice [3]. Animal Model: Intracerebral xenograft model in NCR-Foxn1 mice (female, 7-8 weeks, injected with U251- LUC cells) [3] Dosage: 80 mg/kg Administration: Intraperitoneal injection; every 24 hours for 15 days; monitored tumor progression by BLI on the days 7, 11, 15 Result: Significantly inhibited tumor progression. |
分子量 | 320.39 |
分子式 | C20H20N2O2 |
CAS No. | 1485521-76-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
MOPIPP 1485521-76-3 Inhibitor inhibitor inhibit