Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BAMEAO16B is a lipid nanoparticle that incorporates disulfide bonds for efficient delivery of Cas9 mRNA and sgRNA into cells. In the intracellular environment, BAMEAO16B responds to reduction by releasing RNA, facilitating genome editing. This compound is valuable for gene editing research purposes.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 1,240 | 35日内发货 | ||
10 mg | ¥ 2,340 | 35日内发货 | ||
25 mg | ¥ 5,560 | 35日内发货 | ||
50 mg | ¥ 9,870 | 35日内发货 |
产品描述 | BAMEAO16B is a lipid nanoparticle that incorporates disulfide bonds for efficient delivery of Cas9 mRNA and sgRNA into cells. In the intracellular environment, BAMEAO16B responds to reduction by releasing RNA, facilitating genome editing. This compound is valuable for gene editing research purposes. |
体外活性 | BAMEA-O16B/Cas9 mRNA/sgHPV18 (HeLa cells) treatment significantly prohibits HeLa growth compared to that of a scramble sgRNA and Cas9 mRNA delivery. BAMEA-O16B shows RNA delivery efficiency. BAMEA-O16B shows mRNA encapsulation efficiency. BAMEA-O16B shows GFP knockout efficiency. BAMEA-O16B mediated Cas9mRNA delivery is able to regulate endogenous gene expression. BAMEA-O16B/RNA treated cells shows a higher endosome escape efficiency than that of BAMEA-O16/RNA treatment. BAMEA-O16B/RFP mRNA (HeLa cells) nanoparticles results in efficient RFP expression[1]. |
体内活性 | BAMEA-O16B/Cas9 mRNA/sgRNA (I.v.) nanoparticle effectively knocks mouse serum proprotein convertase subtilisin/kexin type 9 (PCSK9) level down to 20% of nontreated mouse. BAMEA-O16B/Cas9 mRNA/sgPCSK9 nanoparticle reduces mouse serum PCSK9 down to 20% of that with DPBS injection or BAMEA-O16B/Cas9 mRNA/scramblesgRNA nanoparticle injections[1]. |
别名 | BAMEA-O16B |
分子量 | 1114.88 |
分子式 | C56H111N3O6S6 |
CAS No. | 2490668-30-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BAMEA-O16B 2490668-30-7 BAMEA-O-16B BAMEAO16B BAMEA O16B Inhibitor inhibitor inhibit