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Anti-UBC9/UBE2I Antibody (6S11) 是一种 Rabbit 抗体,靶向 UBC9/UBE2I。Anti-UBC9/UBE2I Antibody (6S11) 可用于 WB,ICC/IF,IHC,IP,ChIP。
Anti-UBC9/UBE2I Antibody (6S11) 是一种 Rabbit 抗体,靶向 UBC9/UBE2I。Anti-UBC9/UBE2I Antibody (6S11) 可用于 WB,ICC/IF,IHC,IP,ChIP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-UBC9/UBE2I Antibody (6S11) is a Rabbit antibody targeting UBC9/UBE2I. Anti-UBC9/UBE2I Antibody (6S11) can be used in WB,ICC/IF,IHC,IP,ChIP. |
别名 | ubiquitin-conjugating enzyme E2I, UBC9, P18, C358B7.1 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: HUVEC. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. Counter stained with hematoxylin. 4. ICC staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining UBC9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 18 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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