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Anti-Tryptophan Hydroxylase 1 Antibody (7R847)
还可以产品编号 TMAY-02149
Anti-Tryptophan Hydroxylase 1 Antibody (7R847) 是一种 Rabbit 抗体，靶向 Tryptophan Hydroxylase 1。Anti-Tryptophan Hydroxylase 1 Antibody (7R847) 可用于 WB,IP。
Anti-Tryptophan Hydroxylase 1 Antibody (7R847)
Anti-Tryptophan Hydroxylase 1 Antibody (7R847)
还可以产品编号 TMAY-02149
Anti-Tryptophan Hydroxylase 1 Antibody (7R847) 是一种 Rabbit 抗体，靶向 Tryptophan Hydroxylase 1。Anti-Tryptophan Hydroxylase 1 Antibody (7R847) 可用于 WB,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Tryptophan Hydroxylase 1 Antibody (7R847) is a Rabbit antibody targeting Tryptophan Hydroxylase 1. Anti-Tryptophan Hydroxylase 1 Antibody (7R847) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 7R847 |
| 交叉反应 | Human |
| 验证活性 | 1. TPH1 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg THP-1 Whole Cell Lysate -0.5 µL anti-TPH1 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-TPH1 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 55 kDa. -Observed band size: 60 kDa. 2. Anti-TPH1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: THP1 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:55 kDa. -Observed band size:55 kDa(We are unsure as to the identity of these extra bands.) |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Tryptophan Hydroxylase 1 / TPH1 (rh Tryptophan Hydroxylase 1 / TPH1; TMPY-01959; P17752-1; Ile2-Ile444). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, Tryptophan hydroxylase 1, TPH1, TPH and TPRH, is an enzyme that belongs to the biopterin-dependent aromatic amino acid hydroxylase family. TPH1 contains one ACT domain. Tryptophan hydroxylase catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. TPH1 expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. The tryptophan hydroxylase 1 (TPH1) gene is also reported to be associated with suicidal behavior. Polymorphisms of TPH1 may assist in identifying a subgroup of mood disorder patients that is at higher risk for suicidal behavior. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Tryptophan Hydroxylase 1 / TPH1 Protein (TMPY-01959) |
| 抗原种属 | Human |
| 研究领域 | Neurotransmitter Associated Enzymes |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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