Anti-PPAR gamma/PPARG Antibody (1Z782) is a Mouse antibody targeting PPAR gamma/PPARG. Anti-PPAR gamma/PPARG Antibody (1Z782) can be used in IF,IHC-Fr,IHC-P,WB.

IgG1

1Z782

Human,Mouse (predicted:Rat)

1. Sample:
Eye (Mouse) Lysate at 40 μg
Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
2. Sample:
Lane 1: Liver (Mouse) Tissue Lysate at 40 μg
Lane 2: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 50 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PPAR gamma) Monoclonal Antibody, Unconjugated (TMAB-01567) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human PPAR gamma

Human

PPARG

Peroxisome proliferator-activated receptor gamma

Diabetes associated,Metabolism,Zinc Finger,Fatty acids,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Metabolism,Adipogenesis

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.