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Anti-PPAR gamma/PPARG Antibody (1Z782) 是一种 Mouse 抗体,靶向 PPAR gamma/PPARG。Anti-PPAR gamma/PPARG Antibody (1Z782) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-PPAR gamma/PPARG Antibody (1Z782) 是一种 Mouse 抗体,靶向 PPAR gamma/PPARG。Anti-PPAR gamma/PPARG Antibody (1Z782) 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,160 | 5日内发货 | |
100 μL | ¥ 1,960 | 5日内发货 | |
200 μL | ¥ 2,785 | 5日内发货 |
产品描述 | Anti-PPAR gamma/PPARG Antibody (1Z782) is a Mouse antibody targeting PPAR gamma/PPARG. Anti-PPAR gamma/PPARG Antibody (1Z782) can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG1 |
克隆号 | 1Z782 |
交叉反应 | Human,Mouse (predicted:Rat) |
验证活性 | 1. Sample: Eye (Mouse) Lysate at 40 μg Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa 2. Sample: Lane 1: Liver (Mouse) Tissue Lysate at 40 μg Lane 2: A431 (Human) Cell Lysate at 30 μg Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 50 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PPAR gamma) Monoclonal Antibody, Unconjugated (TMAB-01567) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
亚细胞定位 | Nuclear |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
免疫原 | KLH conjugated synthetic peptide: human PPAR gamma |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Diabetes associated,Metabolism,Zinc Finger,Fatty acids,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Metabolism,Adipogenesis |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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