Anti-Phospho-Tyk2 (Tyr1054, 1055) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Tyk2 (Tyr1054, 1055). Anti-Phospho-Tyk2 (Tyr1054, 1055) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog)

1. Sample:
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-Tyk2 (Tyr1054 + Tyr1055) (TMAB-01526) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kDa
Observed band size: 134 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Phospho-Tyk2 (Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (TMAB-01526) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Phospho-Tyk2 (Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (TMAB-01526) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Tyk2 (Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (TMAB-01526) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Tyk2 around the phosphorylation site of Tyr1054/1055

Human

TYK2

Non-receptor tyrosine-protein kinase TYK2

SARS Coronavirus,Tyrosine Kinases

Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain.