Anti-PARP1 Antibody (5J827) is a Rabbit antibody targeting PARP1. Anti-PARP1 Antibody (5J827) can be used in FCM,WB.

IgG

5J827

Human

25 μg total protein per Lane of various lysates probed with Cleaved PARP monoclonal antibody, unconjugated (TMAB-01333) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:500-2000; FCM: 1:50-100

Monoclonal

A synthesized peptide: human PARP 1

Human

PARP1

Poly [ADP-ribose] polymerase 1

ADP-ribosylation,Base Excision Repair,DNA Damage Recognition

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.