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Anti-NGF Antibody (1M564) 是一种 Rabbit 抗体,靶向 NGF。Anti-NGF Antibody (1M564) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-NGF Antibody (1M564) 是一种 Rabbit 抗体,靶向 NGF。Anti-NGF Antibody (1M564) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
25 μL | ¥ 795 | 5日内发货 | |
50 μL | ¥ 1,390 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-NGF Antibody (1M564) is a Rabbit antibody targeting NGF. Anti-NGF Antibody (1M564) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
Ig Type | IgG |
克隆号 | 1M564 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGF Monoclonal Antibody, Unconjugated (TMAB-01235) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGF Monoclonal Antibody, Unconjugated (TMAB-01235) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGF Monoclonal Antibody, Unconjugated (TMAB-01235) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGF Monoclonal Antibody, Unconjugated (TMAB-01235) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGF Monoclonal Antibody, Unconjugated (TMAB-01235) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGF Monoclonal Antibody, Unconjugated (TMAB-01235) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. 25 μg total protein per Lane of various lysates probed with NGF monoclonal antibody, unconjugated (TMAB-01235) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted. |
构建方式 | Recombinant Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008] |
免疫原 | A synthesized peptide: human beta NGF |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Neurotrophins,Neuroregeneration |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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