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Anti-IKBKB Polyclonal Antibody
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产品编号 TMAB-00930
Anti-IKBKB Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IKBKB。Anti-IKBKB Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-IKBKB Polyclonal Antibody
Anti-IKBKB Polyclonal Antibody
还可以产品编号 TMAB-00930
Anti-IKBKB Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IKBKB。Anti-IKBKB Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-IKBKB Polyclonal Antibody is a Rabbit antibody targeting IKBKB. Anti-IKBKB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. 25 μg total protein per Lane of various lysates probed with IKK beta polyclonal antibody, unconjugated (TMAB-00930) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKK beta Polyclonal Antibody, Unconjugated (TMAB-00930) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKK beta Polyclonal Antibody, Unconjugated (TMAB-00930) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK1. |
| 免疫原 | KLH conjugated synthetic peptide: human IKK beta |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | IKK,Inflammatory mediators,IKK,TLR Signaling,Other Kinases,NFkB Pathway |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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