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Anti-EIF4A1 Antibody (4N444)
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产品编号 TMAC-01271
Anti-EIF4A1 Antibody (4N444) 是一种 Mouse 抗体，靶向 EIF4A1。Anti-EIF4A1 Antibody (4N444) 可用于 WB,IHC,IF。
Anti-EIF4A1 Antibody (4N444)
Anti-EIF4A1 Antibody (4N444)
还可以产品编号 TMAC-01271
Anti-EIF4A1 Antibody (4N444) 是一种 Mouse 抗体，靶向 EIF4A1。Anti-EIF4A1 Antibody (4N444) 可用于 WB,IHC,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,345 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-EIF4A1 Antibody (4N444) is a Mouse antibody targeting EIF4A1. Anti-EIF4A1 Antibody (4N444) can be used in WB,IHC,IF. |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue, using TMAC-01271 diluted at 1:2,000. 2. Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 3. Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 4. Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 5. IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100. 6. Immunofluorescence analysis of Mouse-spleen tissue. 1,eIF4A1 Monoclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-3000; IF: 1:100-200; IHC: 1:50-300 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| 性状 | Liquid |
| 缓冲液 | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| 研究背景 | The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA ¨C this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression. |
| 偶联 | Unconjugated |
| Uniprot ID |
| 分子量 | Actual: 48 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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