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Anti-EIF3K Antibody (9B286)

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产品编号 TMAY-02740

Anti-EIF3K Antibody (9B286) 是一种 Mouse 抗体,靶向 EIF3K。Anti-EIF3K Antibody (9B286) 可用于 ICC/IF。

Anti-EIF3K Antibody (9B286)

Anti-EIF3K Antibody (9B286)

Rating icon 还可以
产品编号 TMAY-02740

Anti-EIF3K Antibody (9B286) 是一种 Mouse 抗体,靶向 EIF3K。Anti-EIF3K Antibody (9B286) 可用于 ICC/IF。

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产品介绍

生物活性
产品描述
Anti-EIF3K Antibody (9B286) is a Mouse antibody targeting EIF3K. Anti-EIF3K Antibody (9B286) can be used in ICC/IF.
Ig Type
Monoclonal Mouse IgG1
克隆号
9B286
交叉反应
Human
验证活性
Immunofluorescence staining of Human EIF3K in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EIF3K monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.
应用
ICC/IF
推荐剂量
ICC-IF: 1:20-1:100
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EIF3K (rh EIF3K; TMPY-03343; Q9UBQ5; Ala2-Gln218). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景EIF3K is a member of the eIF3 subunit K family. It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 4S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. EIF3K is universally expressed in human tissues. It is distributed both in nucleus and cytoplasm. EIF3K is the smallest subunit of eIF3 and it interacts with a number of other subunits of eIF3 and the 4S ribosomal subunit.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human EIF3K Protein (TMPY-03343)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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