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Anti-EIF3K Antibody (9B286) 是一种 Mouse 抗体,靶向 EIF3K。Anti-EIF3K Antibody (9B286) 可用于 ICC/IF。
Anti-EIF3K Antibody (9B286) 是一种 Mouse 抗体,靶向 EIF3K。Anti-EIF3K Antibody (9B286) 可用于 ICC/IF。
产品描述 | Anti-EIF3K Antibody (9B286) is a Mouse antibody targeting EIF3K. Anti-EIF3K Antibody (9B286) can be used in ICC/IF. |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 9B286 |
交叉反应 | Human |
验证活性 | Immunofluorescence staining of Human EIF3K in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EIF3K monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus. |
应用 | ICC/IF |
推荐剂量 | ICC-IF: 1:20-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EIF3K (rh EIF3K; TMPY-03343; Q9UBQ5; Ala2-Gln218). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | EIF3K is a member of the eIF3 subunit K family. It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 4S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. EIF3K is universally expressed in human tissues. It is distributed both in nucleus and cytoplasm. EIF3K is the smallest subunit of eIF3 and it interacts with a number of other subunits of eIF3 and the 4S ribosomal subunit. |
偶联 | Unconjugated |
免疫原 | Recombinant Human EIF3K Protein (TMPY-03343) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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