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Anti-CDK1 Antibody (9C411)
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产品编号 TMAC-00783
别名 P34CDC2, cyclin-dependent kinase 1, CDC28A, CDC2
Anti-CDK1 Antibody (9C411) 是一种 Rabbit 抗体，靶向 CDK1。Anti-CDK1 Antibody (9C411) 可用于 WB,ICC/IF,IHC,IP。
Anti-CDK1 Antibody (9C411)
Anti-CDK1 Antibody (9C411)
还可以产品编号 TMAC-00783 别名 P34CDC2, cyclin-dependent kinase 1, CDC28A, CDC2
Anti-CDK1 Antibody (9C411) 是一种 Rabbit 抗体，靶向 CDK1。Anti-CDK1 Antibody (9C411) 可用于 WB,ICC/IF,IHC,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-CDK1 Antibody (9C411) is a Rabbit antibody targeting CDK1. Anti-CDK1 Antibody (9C411) can be used in WB,ICC/IF,IHC,IP. |
| 别名 | P34CDC2, cyclin-dependent kinase 1, CDC28A, CDC2 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of CDK1 on Jurkat cells lysates using anti-CDK1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDK1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDK1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-CDK1 antibody. Counter stained with hematoxylin. 5. ICC staining CDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CDK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining CDK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cdk1 is a small protein (approximately 34 kilodaltons), and is highly conserved. Cdk1 is comprised mostly by the bare protein kinase motif, which other protein kinases share. Cdk1, like other kinases, contains a cleft in which ATP fits. When bound to its cyclin partners, Cdk1 phosphorylation leads to cell cycle progression. Given its essential role in cell cycle progression, Cdk1 is highly regulated. Most obviously, Cdk1 is regulated by its binding with its cyclin partners. Cyclin binding alters access to the active site of Cdk1, allowing for Cdk1 activity; furthermore, cyclins impart specificity to Cdk1 activity. At least some cyclins contain a hydrophobic patch which may directly interact with substrates, conferring target specificity. Furthermore, cyclins can target Cdk1 to particular subcellular locations. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 34 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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