Anti-CD5 Antibody (1Q411)

Name: Anti-CD5 Antibody (1Q411)
Brand: TargetMol
SKU: TMAY-03826
Rating: 4.5 (1 reviews)

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产品编号 TMAY-03826

Anti-CD5 Antibody (1Q411) 是一种 Mouse 抗体，靶向 CD5。Anti-CD5 Antibody (1Q411) 可用于 FCM,ICC/IF。

Anti-CD5 Antibody (1Q411)

还可以

产品编号 TMAY-03826

Anti-CD5 Antibody (1Q411) 是一种 Mouse 抗体，靶向 CD5。Anti-CD5 Antibody (1Q411) 可用于 FCM,ICC/IF。

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生物活性

产品描述	Anti-CD5 Antibody (1Q411) is a Mouse antibody targeting CD5. Anti-CD5 Antibody (1Q411) can be used in FCM,ICC/IF.
Ig Type	Monoclonal Mouse IgG1
克隆号	1Q411
交叉反应	Rat
验证活性	1. Flow cytometric analysis of Rat CD5 expression on SD rat splenocytes. Cells were stained with purified anti-Rat CD5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of ratCD5 in rat spleen cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with mouse anti-ratCD5 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cell membrane.
应用	FCM,ICC/IF
推荐剂量	ICC-IF: 1:20-1:100; FCM: 1:25-1:100
抗体种类	Monoclonal
宿主来源	Mouse
构建方式	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD5 / Cluster of Differentiation 5 (rR CD5 / Cluster of Differentiation 5; TMPY-03169; P51882; Met1-Pro368). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.

偶联与修饰

偶联	Unconjugated
其他可选修饰符	APC/FITC/PE
可提供抗体种类	4

抗原信息

免疫原	Recombinant Rat CD5 / Cluster of Differentiation 5 Protein (TMPY-03169)
抗原种属	Rat
研究领域	ITIM/ITAM Immunoreceptors and Related Molecules

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.