Anti-Angiopoietin-2 Antibody (7Q134) is a Rabbit antibody targeting Angiopoietin-2. Anti-Angiopoietin-2 Antibody (7Q134) can be used in WB,ICC/IF,IP.

Monoclonal Rabbit IgG

7Q134

Human

1. Immunofluorescence staining of Human Ang2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Ang2 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

2. Anti-Ang2 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell Lysate.

-Lane B: A549 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:57 kDa.

-Observed band size:70 kDa.

3. Ang2 was immunoprecipitated using:

-Lane A:0.5 mg HepG2 Whole Cell Lysate.

-Lane B:0.5 mg A549 Whole Cell Lysate.

-Lane C:0.5 mg 293T Whole Cell Lysate.

-0.5 µL anti-Ang2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-Ang2 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 57 kDa.

-Observed band size: 57 kDa

WB,ICC/IF,IP

WB: 1:500-1:1000; ICC-IF: 1:100-1:500; IP: 0.2-1 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Human Angiopoietin-2 / ANG 2 protein

Human

Cancer Drug Targets