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Anti-ACACA Antibody (3I598) 是一种 Rabbit 抗体,靶向 ACACA。Anti-ACACA Antibody (3I598) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-ACACA Antibody (3I598) 是一种 Rabbit 抗体,靶向 ACACA。Anti-ACACA Antibody (3I598) 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
25 μL | ¥ 790 | 5日内发货 | |
50 μL | ¥ 1,380 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-ACACA Antibody (3I598) is a Rabbit antibody targeting ACACA. Anti-ACACA Antibody (3I598) can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
克隆号 | 3I598 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by microwaving in EDTA buffer (pH8.0) for 5 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Acetyl CoA Carboxylase 1 (ACC1) Monoclonal Antibody, Unconjugated (TMAB-00029) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwaving in EDTA buffer (pH8.0) for 5 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Acetyl CoA Carboxylase 1 (ACC1) Monoclonal Antibody, Unconjugated (TMAB-00029) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Mouse Brain Lysates Lane 2: Human K562 cell Lysates Primary: Anti-Acetyl CoA Carboxylase 1 (ACC1) (TMAB-00029) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 265 kDa Observed band size: 265 kDa |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cytoplasm. |
构建方式 | Recombinant Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
免疫原 | KLH conjugated synthetic peptide: human Acetyl CoA Carboxylase 1(ACC1) |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Integration of energy metabolism,Metabolism of lipids and lipoproteins,Synthesis,Integration of energy,Lipid metabolism,Mitochondrial markers,Fatty acid oxidation,Lipid metabolism,Mitochondrial |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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