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Anti-4EBP1 Antibody (9E210)
还可以产品编号 TMAY-00090
Anti-4EBP1 Antibody (9E210) 是一种 Mouse 抗体，靶向 4EBP1。Anti-4EBP1 Antibody (9E210) 可用于 WB,ELISA,ICC/IF。
Anti-4EBP1 Antibody (9E210)
Anti-4EBP1 Antibody (9E210)
还可以产品编号 TMAY-00090
Anti-4EBP1 Antibody (9E210) 是一种 Mouse 抗体，靶向 4EBP1。Anti-4EBP1 Antibody (9E210) 可用于 WB,ELISA,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-4EBP1 Antibody (9E210) is a Mouse antibody targeting 4EBP1. Anti-4EBP1 Antibody (9E210) can be used in WB,ELISA,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 9E210 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human 4EBP1 monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm. 2. Anti-4EBP1 mouse monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell Lysate. -Lane B: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:13 kDa. -Observed band size:13 kDa |
| 应用 | WB,ELISA,ICC/IF |
| 推荐剂量 | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; ICC-IF: 1:100-1:500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 4E-BP1 / EIF4EBP1 (rh 4E-BP1 / EIF4EBP1; TMPY-01769; Ser 2-Ile 118; NP_004086.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography . |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The translational suppressor eIF4E binding protein-1, 4E-BP1 functions as a key regulator in cellular growth, differentiation, apoptosis and survival. The Eif4ebp1 gene, encoding 4E-BP1, is a direct target of a transcription factor activating transcription factor-4 (ATF4), a master regulator of gene expression in stress responses. 4E-BP1 is characterized by its capacity to bind specifically to eIF4E and inhibit its interaction with eIF4G. Phosphorylation of 4E-BP1 regulates eIF4E availability, and therefore, cap-dependent translation, in cell stress. Binding of eIF4E to eIF4G is inhibited in a competitive manner by 4E-BP1. Phosphorylation of 4E-BP1 decreases the affinity of this protein for eIF4E, thus favouring the binding of eIF4G and enhancing translation. 4E-BP1 is important for beta-cell survival under endoplasmic reticulum (ER) stress. 4E-BP1 mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recently, 4E-BP1 was found to be a key factor, which converges several oncogenic signals, phosphorylates the molecules, and drives the downstream proliferative signals. Recent studies showed that high expression of phosphorylated 4E-BP-1 (p-4E-BP1) is associated with poor prognosis, tumor progression, or nodal metastasis in different human cancers. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human 4E-BP1 / EIF4EBP1 Protein (TMPY-01769) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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