Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Chalcone (Cinnamophenone) 是从甘草中分离得到的一种芳香酮,可合成查耳酮衍生物。它具有多种生物和药理的活性,如抗炎、抗菌、抗癌、抗氧化、抗寄生虫等活性。
产品描述 | Chalcone (Cinnamophenone) is an aromatic ketone that forms the central core for a variety of important biological compounds, which are known collectively as chalcones. |
靶点活性 | EGFR:10 µM (in A431 cells) |
体外活性 | AG99, a potent inhibitor of EGFR, was shown to inhibit the appearance of pp145 to some extent. AG99 promotes cell death of normal and cancer cells via activation of caspase-like proteases in vitro [2]. |
细胞实验 | Human bladder carcinoma cell line 5637 was maintained in RPMI1640 medium supplemented with 10% fetal calf serum (FCS), 1 mM L-glutamine, and antibiotics. Cells were grown at 37°C in a humidified 5% CO2 atmosphere. For all experiments, confluent cells were trypsinized, and aliquots of single-cell suspension were re-seeded at 0.5×10^6 cells/dish (100-mm diameter) or 0.1×106 cells/dish (60-mm diameter) and cultured in poly-L-lysine-coated new culture dishes containing normal culture media for 24-48 hours. After the normal growth treatment, cells were treated under several different conditions as specified in the text. When cell number and cell viability were determined, both attached cells and unattached cells were collected by trypsinization and sedimentation of the culture media, respectively, and combined in one tube. The cells were washed twice with phosphate-buffered saline (PBS) and aliquots of the cells were stained with Trypan Blue solution. After the staining, cells were mounted in a hemocytometer for microscopic observation. Total cell number was determined by counting more than 300 cells per condition. The percentage of Trypan-Blue-stained cells (versus total cell number) was determined by calculation of data obtained with three independent counting of more than 100 cells. When nuclear morphology was evaluated, cells were grown and treated in glass-bottom dishes. After treatment, the cells were washed three times with PBS, fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.2% Triton X-100 in PBS for 2 minutes and blocked with 3% BSA in PBS for 1 hour. Cells were then treated with propidium iodide at 2 μg/ml in PBS for 1 hour. After washing with PBS (three times 5 minutes), samples were mounted in PBS containing 50 mg/ml 1,4-diazabicyclo[2,2,2]octane and 90% glycerol and subjected to confocal microscopic observation. The fluorescence was detected at 543 nm argon excitation using optics of a 590 nm long-pass barrier filter.The fluorescent images were recorded and reconstituted with the use of software Adobe Photoshop CS [2]. |
别名 | benzylideneacetophenone, phenyl styryl ketone, Cinnamophenone, benzalacetophenone, β-phenylacrylophenone, 查耳酮 |
分子量 | 208.26 |
分子式 | C15H12O |
CAS No. | 94-41-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 201.7 mM
H2O: 4.8 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 4.8017 mL | 24.0085 mL | 48.0169 mL | 120.0423 mL |
DMSO | 5 mM | 0.9603 mL | 4.8017 mL | 9.6034 mL | 24.0085 mL |
10 mM | 0.4802 mL | 2.4008 mL | 4.8017 mL | 12.0042 mL | |
20 mM | 0.2401 mL | 1.2004 mL | 2.4008 mL | 6.0021 mL | |
50 mM | 0.096 mL | 0.4802 mL | 0.9603 mL | 2.4008 mL | |
100 mM | 0.048 mL | 0.2401 mL | 0.4802 mL | 1.2004 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Chalcone 94-41-7 Angiogenesis JAK/STAT signaling Microbiology/Virology Tyrosine Kinase/Adaptors EGFR Antibacterial anti-parasitic anti-inflammatory b-phenylacrylophenone beta-phenylacrylophenone benzylideneacetophenone phenyl styryl ketone antibacterial Cinnamophenone Inhibitor Bacterial antioxidative benzalacetophenone β-phenylacrylophenone anticancer 查耳酮 inhibit inhibitor