Anti-EGFR Polyclonal Antibody 3 is a Rabbit antibody targeting EGFR. Anti-EGFR Polyclonal Antibody 3 can be used in WB,IHC-Fr,IF,ELISA,IHC-P.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (TMAB-00597) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (TMAB-00597) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (TMAB-00597) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human MDA-MB-231 cell lysates
Primary: Anti-EGFR (TMAB-00597) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa
5. 25 μg total protein per Lane of various lysates probed with EGFR polyclonal antibody, unconjugated (TMAB-00597) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000; IHC-P: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human EGFR

Human

EGFR

Epidermal growth factor receptor

Receptor Tyrosine Kinases,EGF,egfr,SARS Coronavirus,Cell differentiation,Receptors,Receptor tyrosine kinases,Growth factor receptors,EGF

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.