5a-Pregnane-3,20-dione is a biologically active 5-alpha-reduced metabolite of plasma progesterone

MCF-7 cells were grown without or with 5a-Pregnane-3,20-dione (10(-9)-10(-5) M), and the effects on cell and nuclear morphology, adhesion plaques, vinculin and actin expression, actin polymerization, and microfilament distribution were examined by immunohistochemistry, morphometry (using confocal microscopy and digital computer imaging analysis), and Western blotting.?Treatment of cells with 10(-9)-10(-6) M 5a-Pregnane-3,20-dione resulted in dose-dependent decreases in cell area, cell-to-cell contacts, and attachment to the substratum, and increases in variation in nuclear area.?These changes in the 5alphaP-treated cells were accompanied by decreases in vinculin-containing adhesion plaques, vinculin expression, polymerized actin stress fibers, and decreases in insoluble and increases in soluble actin fractions.

氢化黄体酮

DMSO: 1.24 mg/mL (3.92 mM), Sonication is recommended.

10% DMSO+90% Saline: 0.12 mg/mL (0.38 mM), Solution.