Name: 3,6-DMAD hydrochloride
Brand: TargetMol
SKU: T10102
Rating: 4.5 (1 reviews)

规格

价格

库存

数量

25 mg

待询

3-6月

50 mg

待询

3-6月

100 mg

待询

3-6月

生物活性

产品描述	3,6-DMAD hydrochloride functions as an inhibitor for the IRE1α-XBP1 pathway within the unfolded protein response mechanism.
体外活性	3,6-DMAD inhibits both IRE1α oligomerization and in vitro endoribonuclease (RNase) activity.
体内活性	3,6-DMAD (10 mg/kg, i.p., once every 12 h, total thrice) significantly inhibits in vivo XBP1-luciferase activity assessed 3.5 days after the initial treatment. 3,6-DMAD-treatment significantly inhibits tumor xenograft growth.
激酶实验	IRE1α oligomerization assay is set up using the nuclease reaction buffer, 2 μM recombinant hIRE1α, 2 mM ADP, and 60 μM of 3,6-DMAD. All reactions are performed in 20 μL with 10% DMSO to account for the vehicle and incubated for 15 min at 30 °C to allow for oligomerization. The optical density of the sample is measured at 500 nm using a NanoDrop 2000.
细胞实验	2×10^4 cells per well are plated into 96-well plates and treatment started 0-12 hours after plating. RPMI 8226 and MM1.R human MM cells are treated with 0-6 μM 3,6-DMAD. After 24 hours of treatment, XTT reagent (ATCC) is added to the wells then cells are incubated for 2 hours and absorbance measured at both 475 nM and 660 nM using a plate reader.
动物实验	5×10^6 RPMI 8226 cells are implanted subcutaneously into the flanks of 4-6 weeks' old NOD Scid mice. Drug treatment started when the sizes of the tumors reached 150 mm3. Four tumor-bearing mice per group are treated with 10 mg/kg 3,6-DMAD or vehicle (saline) intraperitoneally once every 2 days.

化学信息

分子量	365.52
分子式	C₂₂H₃₁N₅xHCl
Smiles	CN(C1=CC=C2C(N=C3C=C(C=CC3=C2NCCCN(C)C)N(C)C)=C1)C.Cl
密度	1.31g/cm3

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

DMSO: 25 mg/mL (68.4 mM), Sonication is recommended.

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7358 mL	13.6791 mL	27.3583 mL	136.7914 mL
5 mM	0.5472 mL	2.7358 mL	5.4717 mL	27.3583 mL
10 mM	0.2736 mL	1.3679 mL	2.7358 mL	13.6791 mL
20 mM	0.1368 mL	0.6840 mL	1.3679 mL	6.8396 mL
50 mM	0.0547 mL	0.2736 mL	0.5472 mL	2.7358 mL