TMED1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.6 kDa and the accession number is Q13445.
生物活性 | 1.Measured by its binding ability in a functional ELISA. 2. Immobilized human TMED1-His at 10μg/mL (100μL/well) can bind human IL1R4-Fc, the EC50 of human IL1R4-Fc is 8-50ng/mL. |
产品描述 | TMED1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.6 kDa and the accession number is Q13445. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q13445 |
别名 | Tp24, transmembrane emp24 protein transport domain containing 1, IL1RL1LG, Il1rl1l |
蛋白构建 | The Human TMED1 (Q13445) (Met1-Asn194) was expressed with a polyhistidine tag at the C-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 20.6 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side. |
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TMED1 Protein, Human, Recombinant (His) Tp24 transmembrane emp24 protein transport domain containing 1 IL1RL1LG Il1rl1l recombinant recombinant-proteins proteins protein