TMED1 重组蛋白, Human (His)

TMED1 Protein, Human, Recombinant (His)

产品编号 TMPY-03119

TMED1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.6 kDa and the accession number is Q13445.

TMED1 Protein, Human, Recombinant (His)

产品编号 TMPY-03119

TMED1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.6 kDa and the accession number is Q13445.

规格	价格	库存	数量
100 μg	¥ 4,460	5日内发货

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常见问题解答

问如何设置母液浓度？

答母液浓度需低于官网给出的溶解度，在这个范围内，根据工作液浓度设置母液浓度，细胞实验中，建议母液浓度设定在工作液浓度的 1000 倍以上。

问细胞实验中，抑制剂如何溶解？

答用 DMSO 配置较高浓度母液，母液存于 -80℃，建议多分装几管，一周内使用的存于 4℃，避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释，细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%，则需要在做预试验的时候，先进行溶剂阴性对照实验，排除溶剂对细胞的影响。

问动物不能耐受 DMSO，给药时 DMSO量应该如何控制？

答对于普通的老鼠，DMSO 的浓度应控制在 10% 以下，对于裸鼠、转基因小鼠、耐受性弱的老鼠等，DMSO 浓度需控制在2%以下。对于首次操作的抑制剂，建议先做溶剂阴性对照，确认溶解对动物无非特异性影响。

问超声久了会不会对化合物的结构有影响？

答建议以较低频率对化合物进行超声，不会对化合物的结构造成影响。

问收到粉状试剂粘壁严重，如何处理？

答建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。

产品信息

生物活性	1.Measured by its binding ability in a functional ELISA. 2. Immobilized human TMED1-His at 10μg/mL (100μL/well) can bind human IL1R4-Fc, the EC50 of human IL1R4-Fc is 8-50ng/mL.
产品描述	TMED1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.6 kDa and the accession number is Q13445.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q13445
别名	transmembrane emp24 protein transport domain containing 1,Tp24,IL1RL1LG,Il1rl1l
蛋白构建	A DNA sequence encoding the human TMED1 (Q13445) (Met1-Asn194) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 24
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	20.6 kDa (predicted); 28 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.

TMED1 Protein, Human, Recombinant (His)

TMED1 Protein, Human, Recombinant (His)

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