TIMP-1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 21 kDa and the accession number is P01033.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | ¥ 2,530 | 现货 | |
| 100 μg | ¥ 8,970 | 5日内发货 |
| 生物活性 | Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2. The IC50 value is < 6 nM. |
| 产品描述 | TIMP-1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 21 kDa and the accession number is P01033. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | Tag Free |
| 蛋白编号 | P01033 |
| 别名 | TIMP-1,TIMP metallopeptidase inhibitor 1,TIMP,HCI,EPO,EPA,CLGI |
| 蛋白构建 | A DNA sequence encoding the mature form of human TIMP1 (NP_003245.1) (Cys 24-Ala 207) was expressed. Predicted N terminal: Cys 24 |
| 蛋白纯度 | > 97 % as determined by SDS-PAGE  |
| 分子量 | 21 kDa (predicted); 26 kDa (reducing conditions) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM NaAC, 200 mM NaCl, pH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | TIMP metallopeptidase inhibitor 1, also known as TIMP-1/TIMP1, Collagenase inhibitor 16C8 fibroblast Erythroid-potentiating activity, TPA-S1TPA-induced proteinTissue inhibitor of metalloproteinases 1, is a natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. TIMP-1/TIMP1 is found in fetal and adult tissues. Highest levels are found in bone, lung, ovary and uterus. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. In addition to its inhibitory role against most of the known MMPs, the protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this protein encoding gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This encoding gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 is Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. |
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