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SLPI Protein, Human, Recombinant (His)

产品编号 TMPY-02389

SLPI Protein, Human, Recombinant (His) is expressed in Baculovirus-Insect Cells. The accession number is P03973.

SLPI Protein, Human, Recombinant (His)

SLPI Protein, Human, Recombinant (His)

产品编号 TMPY-02389
SLPI Protein, Human, Recombinant (His) is expressed in Baculovirus-Insect Cells. The accession number is P03973.
规格价格库存数量
100 μg
¥ 3,820
5日内发货
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细胞实验中,抑制剂如何溶解?
用 DMSO 配置较高浓度母液,母液存于 -80℃,建议多分装几管,一周内使用的存于 4℃,避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释,细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%,则需要在做预试验的时候,先进行溶剂阴性对照实验,排除溶剂对细胞的影响。
动物实验常用溶解方法?
首先,您需要确认给药剂量、给药方式。对于具体的产品,优先找引用我们产品的文献里的使用方法,再找其他文献里的配方。 如果没有相关的文献,且该化合物的 DMSO 溶解度比较好,我们推荐通用配方: 10% DMSO+40% PEG300+5% Tween-80+45% Saline/PBS/ddH2O。溶剂依次加入,尽量溶解后再加入下一个溶剂。正常鼠建议 DMSO 浓度在 10% 以下,裸鼠体弱鼠等配方的 DMSO 浓度建议在 2% 以下,根据溶液是否澄清,助溶剂 PEG300、Tween-80 的比例可以适当调整。如果有其他助溶剂也可以使用。 以上配方仅供参考,体内配方并不是绝对的,需要根据不同情况进行调整。建议先取少量化合物测试配方,再进行大量配制。配完也可以再使用超声、加热等方式助溶,看看能不能澄清一点。 腹腔注射对粉末的溶解度要求比较高,建议购买盐形式化合物。如果给药剂量比较大,也有报道使用混悬液进行腹腔给药的。 对于灌胃给药,且剂量比较大的情况下,建议用 0.5% CMC-Na 配置成均匀混悬液进行给药。
购买多次产品,发现颜色不同,怎么办?
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如果低于说明书给出的最高溶解度情况下,产品仍未溶解怎么办?
建议您使用一些辅助溶解方法,如水浴加热至 45℃ 或用超声震荡,加速粉末溶解。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
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产品信息

生物活性
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2 (Catalog # ES002).The IC50 value is <1 nM.
产品描述
SLPI Protein, Human, Recombinant (His) is expressed in Baculovirus-Insect Cells. The accession number is P03973.
种属
Human
表达系统
Baculovirus Insect Cells
标签C-His
蛋白编号P03973
别名
WFDC4,WAP4,MPI,HUSI-I,HUSI,BLPI,ALP,ALK1
蛋白构建
A DNA sequence encoding the human SLPI (P03973)( Met1-Ala132) was expressed, with a C-terminal polyhistidine tag.
蛋白纯度
≥ 90% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.
分子量13.17 kDa (predicted); 15 kDa (reducing contition, due to glycosylation)
内毒素< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% gly, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法
Please refer to the lot-specific COA.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Secretory leukoprotease inhibitor (SLPI), also called antileukoprotease (ALP), is a 12-kDa, nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells, including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites, and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract, possessing serine protease inhibitor activity, antibacterial activity, and anti-inflammatory/immunomodulatory activity.

参考文献

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