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RPLP0 Protein, Mouse, Recombinant (His & Myc & SUMO)

产品编号 TMPH-02483RPLP0 Protein, Mouse, Recombinant (His & Myc & SUMO)

Ribosomal protein P0 is the functional equivalent of E.coli protein L10. RPLP0 Protein, Mouse, Recombinant (His & Myc & SUMO) is expressed in E. coli expression system with N-10XHis-SUMO and C-Myc tag. The predicted molecular weight is 54.2 kDa and the accession number is P14869.

RPLP0 Protein, Mouse, Recombinant (His & Myc & SUMO)

RPLP0 Protein, Mouse, Recombinant (His & Myc & SUMO)

产品编号 TMPH-02483RPLP0 Protein, Mouse, Recombinant (His & Myc & SUMO)
Ribosomal protein P0 is the functional equivalent of E.coli protein L10. RPLP0 Protein, Mouse, Recombinant (His & Myc & SUMO) is expressed in E. coli expression system with N-10XHis-SUMO and C-Myc tag. The predicted molecular weight is 54.2 kDa and the accession number is P14869.
规格价格库存数量
20 μg
¥ 1,800
20日内发货
100 μg
¥ 3,760
20日内发货
1 mg
¥ 16,000
20日内发货
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TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
抑制剂母液的储存方式?
母液配置完成后,一般储存于 -80℃,可以存 1 年以上。建议多分装几管,避免反复冻融,常用的存于 4 度,可放一周以上。
是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释?
大部分情况下,都是可以溶解的。但有时,有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释,再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。 比如细胞实验中希望终浓度为 1 μM 的话,可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM,再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液,终浓度即为 1 μM。 为避免药物析出,稀释前,可将母液和培养基 37℃ 预热,避免温度低造成严重析出。若稀释过程中出现化合物析出的情况,建议您采用超声加热的方法使其复溶。
化合物如何用于动物实验?如何确立剂量、给药方式等?
同一个化合物对不同动物模型、不同疾病模型的用法不一样,建议根据实验目的,查询文献选择用法。 常见的给药方式包括腹腔注射、灌胃等。由于首过效应,一般可以灌胃的也可以腹腔注射,可以腹腔注射的不一定适合灌胃。不同种属的给药剂量可以根据官网的表格换算。但仅供参考,实际应用中可能未必合适或者准确,尽量以具体某种动物给药剂量的参考文献为主。 我们的化合物都可以进行细胞动物实验,但有些化合物还没有用于动物实验的文献,这种情况下,您可以尝试进行动物实验,但我们无法提供使用方式,也无法保证药效。
产品是否需要避光?
一般来说,如果有需要避光储存的分子,我们会选择棕色玻璃瓶发货的。
超声应使用什么仪器?
建议使用“超声清洗仪”,不推荐用“超声破碎仪”。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Ribosomal protein P0 is the functional equivalent of E.coli protein L10. RPLP0 Protein, Mouse, Recombinant (His & Myc & SUMO) is expressed in E. coli expression system with N-10XHis-SUMO and C-Myc tag. The predicted molecular weight is 54.2 kDa and the accession number is P14869.
种属
Mouse
表达系统
E. coli
标签N-10XHis-SUMO, C-Myc
蛋白编号P14869
别名
Rplp0,Large ribosomal subunit protein uL10,Arbp,60S ribosomal protein L10E,60S acidic ribosomal protein P0
氨基酸序列
MPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLIIQQVFDNGSIYNPEVLDITEQALHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETEYTFPLTEKVKAFLADPSAFAAAAPAAAATTAAPAAAAAPAKAEAKEESEESDEDMGFGLFD
蛋白构建
1-317 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量54.2 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Tris-based buffer, 50% glycerol
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Ribosomal protein P0 is the functional equivalent of E.coli protein L10.

参考文献

引用文献

1.Tan X, Yang Y, Xu J, et al. Luteolin Exerts Neuroprotection via Modulation of the p62/Keap1/Nrf2 Pathway in Intracerebral Hemorrhage. Frontiers in Pharmacology. 2020, 10: 15512.Yang Y, Tan X, Xu J, et al. Luteolin alleviates neuroinflammation via downregulating the TLR4/TRAF6/NF-κB pathway after intracerebral hemorrhage. Biomedicine & Pharmacotherapy. 2020, 126: 1100443.Zhang Y, Yu F, Hao J, et al. Study on the Effective Material Basis and Mechanism of Traditional Chinese Medicine Prescription (QJC) Against Stress Diarrhea in Mice. Frontiers in Veterinary Science. 2021, 8.4.Ding X, Zheng L, Yang B, et al. Luteolin Attenuates Atherosclerosis Via Modulating Signal Transducer And Activator Of Transcription 3-Mediated Inflammatory Response. Drug Des Devel Ther. 2019, 13: 3899.5.Hwang Y H, Jang S A, Kim T, et al. Anti-osteoporotic and Anti-adipogenic Effects of Rhus chinensis Nutgalls in Ovariectomized Mice Fed with a High-fat Diet. Planta Medica. 2019, 85(14/15): 1128-11356.Xiao Z, Xu H, Qu Z, et al. Active Ingredients of Reduning Injections Maintain High Potency against SARS-CoV-2 Variants. Chinese Journal of Integrative Medicine. 2022: 1-8.7.Dong J, Xu O, Wang J, et al.  Luteolin ameliorates inflammation and Th1/Th2 imbalance via regulating the TLR4/NF-κB pathway in allergic rhinitis rats. Immunopharmacology and Immunotoxicology. 2021 Jun;43(3):319-327. doi: 10.1080/08923973.2021.1905659. Epub 2021 Apr 26.8.Jung M A, Song H K, Jo K, et al.Gleditsia sinensis Lam. aqueous extract attenuates nasal inflammation in allergic rhinitis by inhibiting MUC5AC production through suppression of the STAT3/STAT6 pathway.Biomedicine & Pharmacotherapy.2023, 161: 114482.9.Zhu Y, Bao G, Zhu G, et al.Discovery and characterization of natural product luteolin as an effective inhibitor of human pyridoxal kinase.Bioorganic Chemistry.2023: 107057.10.Yu S, Wu R, Si Y, et al.Alternative splicing of ALDOA confers tamoxifen resistance in breast cancer.Oncogene.2024: 1-13.11.Hoser J, Weglinska G, Samsel A, et al.Modulation of the Respiratory Epithelium Physiology by Flavonoids—Insights from 16HBEσcell Model.International Journal of Molecular Sciences.2024, 25(22): 11999.

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