Pepsinogen C/PGC Protein, Human, Recombinant (aa 153-239, His) is expressed in E. coli. The accession number is P20142-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μg | ¥ 3,820 | 期货 |
| 生物活性 | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| 产品描述 | Pepsinogen C/PGC Protein, Human, Recombinant (aa 153-239, His) is expressed in E. coli. The accession number is P20142-1. |
| 种属 | Human |
| 表达系统 | E. coli |
| 标签 | N-His |
| 蛋白编号 | P20142-1 |
| 别名 | PGII,PEPC |
| 蛋白构建 | A DNA sequence encoding the mature form of human PGC (P20142-1) (Ile153-Ile239) was expressed with a polyhistide tag at the N-terminus. |
| 蛋白纯度 | > 95% as determined by SDS-PAGE |
| 分子量 | 10.9 kDa (predicted); 10 kDa (reducing contition) |
| 内毒素 | Please contact us for more information. |
| 缓冲液 | Lyophilized from sterile 50mM Tris, 0.4M sucrose, 1mM EDTA, 50mM NaCl, pH 8.0Pleasecontact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 复溶方法 | Please refer to the lot-specific COA. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. At low PH conditions, Pepsinogen C undergoes conversion into active enzyme. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. |
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