NEDD8 Protein, Human, Recombinant

Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation. NEDD8 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 9 KDa and the accession number is Q15843.

规格	价格/CNY		数量
10 μg	￥ 80	5日内发货
50 μg	￥ 280	5日内发货
500 μg	￥ 1,270	5日内发货
1 mg	￥ 1,920	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 80

5日内发货

50 μg

￥ 280

5日内发货

500 μg

￥ 1,270

5日内发货

1 mg

￥ 1,920

5日内发货

产品描述

种属	Human
表达系统	E. coli
标签	Tag Free
蛋白编号	Q15843
别名	Ubiquitin-like protein Nedd8, NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8
氨基酸序列	Met1-Gly76
蛋白构建	Met1-Gly76
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	9 KDa (reducing condition)

内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 250 mM NaCl, pH 8.0.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

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NEDD8 Protein, Human, Recombinant

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