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Insulin Protein, Bovine, Recombinant

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货号 TMPS-00095

别名 Insulin

Insulin Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is P01317.

Insulin Protein, Bovine, Recombinant

Insulin Protein, Bovine, Recombinant

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Rating icon 还可以
货号 TMPS-00095 别名 Insulin

Insulin Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is P01317.

规格价格库存数量
25 mg
¥ 2,390
5日内发货
50 mg
¥ 3,950
5日内发货
100 mg
¥ 6,830
5日内发货
200 mg
¥ 11,200
5日内发货
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产品介绍


生物活性
产品描述
Insulin Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is P01317.
生物活性
ED 50 < 1.00 μg/ml as determined by a dose-response proliferation assay using human MCF7 epithelial cells. Based on the ED 50 , the calculated specific activity is approximately > 1× 10 3 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.
研究背景
Insulin is a peptide hormone produced exclusively by beta cells of the pancreatic islets. The mature form of insulin is a heterodimer of a B chain and an A chain linked by two disulfide bonds. The amino acid sequence of insulin is strongly conserved and varies only slightly between species. Bovine insulin differs from human in only three amino acid residues, and porcine insulin in one. Insulin is considered to be the main anabolic hormone of the body and regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of glucose from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes. Recombinant insulin has been widely used for treatment of diabetes.
种属
Bovine
表达系统
P. pastoris (Yeast)
标签Tag Free
蛋白编号P01317
蛋白构建Phe25-Ala54 & Gly85-Asn105
蛋白纯度
≥ 95% as determined by SDS-PAGE
缓冲液Lyophilized from a 0.2 μm filtered solution in 10 mM Citric acid, pH 2.0-3.0.
复溶方法Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH 2 O up to 1 mg/ml.
别名Insulin
内毒素< 0.1 EU/μg of protein as determined by the LAL method.
化学信息
分子量5.72 kDa (Predicted); 6 kDa (Reducing conditions)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

剂量转换

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技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.
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