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Influenza A H4N8 (A/chicken/Alabama/1/1975) Hemagglutinin/HA1 Protein (His)

产品编号 TMPY-02619

Influenza A H4N8 (A/chicken/Alabama/1/1975) Hemagglutinin/HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.6 kDa and the accession number is P19695.1.

Influenza A H4N8 (A/chicken/Alabama/1/1975) Hemagglutinin/HA1 Protein (His)

Influenza A H4N8 (A/chicken/Alabama/1/1975) Hemagglutinin/HA1 Protein (His)

产品编号 TMPY-02619
Influenza A H4N8 (A/chicken/Alabama/1/1975) Hemagglutinin/HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.6 kDa and the accession number is P19695.1.
规格价格库存数量
100 μg
¥ 4,460
5日内发货
大包装 & 定制
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实验操作小课堂
Influenza A H4N8 (A/chicken/Alabama/1/1975) Hemagglutinin/HA1 Protein (His)产品问题解答
请问 DAPT 粉末加生理盐水和 PEG300 加超声也无法做成很好的混悬液,实验是病鼠,加 DMSO 的话比例又不能太大,比例小量小溶不进去,量大了又不行,可以灌胃或雾化吸入吗?
可以尝试用灌胃,可以使用环糊精,环糊精溶解一般会比较均匀,参考文献:γ‐secretase inhibitor DAPT mitigates cisplatin‐induced acute kidney injury by suppressing Notch1 signaling;DAPT, a γ-Secretase Inhibitor, Suppresses Tumorigenesis, and Progression of Growth Hormone-Producing Adenomas by Targeting Notch Signaling。
常见问题解答
动物实验常用溶解方法?
首先,您需要确认给药剂量、给药方式。对于具体的产品,优先找引用我们产品的文献里的使用方法,再找其他文献里的配方。 如果没有相关的文献,且该化合物的 DMSO 溶解度比较好,我们推荐通用配方: 10% DMSO+40% PEG300+5% Tween-80+45% Saline/PBS/ddH2O。溶剂依次加入,尽量溶解后再加入下一个溶剂。正常鼠建议 DMSO 浓度在 10% 以下,裸鼠体弱鼠等配方的 DMSO 浓度建议在 2% 以下,根据溶液是否澄清,助溶剂 PEG300、Tween-80 的比例可以适当调整。如果有其他助溶剂也可以使用。 以上配方仅供参考,体内配方并不是绝对的,需要根据不同情况进行调整。建议先取少量化合物测试配方,再进行大量配制。配完也可以再使用超声、加热等方式助溶,看看能不能澄清一点。 腹腔注射对粉末的溶解度要求比较高,建议购买盐形式化合物。如果给药剂量比较大,也有报道使用混悬液进行腹腔给药的。 对于灌胃给药,且剂量比较大的情况下,建议用 0.5% CMC-Na 配置成均匀混悬液进行给药。
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
加药后检测结果没有作用,是不是产品质量有问题?
产品质量没有问题,都是经过两次严格质检的,HNMR确定产品结构,HPLC确定化合物纯度。同一个抑制剂由于实验材料(细胞、动物)的不同,IC50 和实验效果也是不一样的,不能照搬文献报道的方法,应该根据自己的实验设立浓度梯度,得到一个最适合的浓度。
Tween-20 可以用 Tween-80 替代吗?
不建议使用 Tween-20。相较于 Tween-20,Tween-80 的耐受性更好,Tween-80已被用作评估实验药物和毒物的行为影响的工具,且没有明显的副作用。 参考文献:Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
细胞实验中需要加多少抑制剂?
通常建议您参考同模型实验发表的文献报道;除参考文献报道以外,还需要通过预实验做“剂量-效应曲线”确定最佳作用浓度(浓度梯度);通过“时间-效应曲线”确定最佳孵育时间(处理时间梯度)。另外,抑制剂的使用量受多种因素影响,包括抑制对象的可接触性、细胞通透性、孵育时间、细胞种类等等。我们建议通过检索文献确定使用抑制剂的起始浓度。 如果有报道的 Ki 值或 IC50 值,可以采用其 5-10 倍的量开始尝试以达到抑制酶活性的最佳效果。 如果抑制剂的 Ki 值或 IC50 值未知,则需要在更广泛的范围尝试抑制剂的使用浓度,并采用 Michaelis-Menten 动力学计算 Ki 值。 一般设立溶解抑制剂时所采用的溶剂作为对照,以排除溶剂的非特异性影响。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Influenza A H4N8 (A/chicken/Alabama/1/1975) Hemagglutinin/HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.6 kDa and the accession number is P19695.1.
种属
H4N8
表达系统
HEK293 Cells
标签C-His
蛋白编号P19695.1
别名
Harvey rat sarcoma viral oncogene homolog
蛋白构建
A DNA sequence encoding the N-terminal segment (Met 1-Arg 343) of the influenza hemagglutinin (A/chicken/Alabama/1/1975 (H4N8)) (P19695.1), termed as HA1, was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 17
蛋白纯度
> 97 % as determined by SDS-PAGE
分子量37.6 kDa (predicted); 55 kDa (reducing condition, due to glycosylation)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

参考文献

1.Jiang LY, et al. γ-Secretase Inhibitor, DAPT Inhibits Self-renewal and Stemness Maintenance of Ovarian Cancer Stem-like Cells In Vitro. Chin J Cancer Res. 2011 Jun;23(2):140-6.2.Feng J, et al. DAPT, a γ-Secretase Inhibitor, Suppresses Tumorigenesis, and Progression of Growth Hormone-Producing Adenomas by Targeting Notch Signaling. Front Oncol. 2019 Aug 27;9:809.3.Soni H, et al. γ-secretase inhibitor DAPT mitigates cisplatin-induced acute kidney injury by suppressing Notch1 signaling. J Cell Mol Med. 2019 Jan;23(1):260-270.4.Majumder S, et al. Shifts in podocyte histone H3K27me3 regulate mouse and human glomerular disease. J Clin Invest. 2018 Jan 2;128(1):483-499.5.Yixin Tao, et al. β-catenin activation in hair follicle dermal stem cells induces ectopic hair outgrowth and skin fibrosis. J Mol Cell Biol. 2018 May 16.6.Zhou Y, Li X. LxA4 Inhibits LPS Induced Proinflammatory Response and Microglia Polarization through Notch Signaling Pathway[J]. Revista Argentina de Clínica Psicológica. 2021, 30(1): 330.7.Huang C, Yan T, Xu Z, et al. Hypoxia Enhances Fusion of Oral Squamous Carcinoma Cells and Epithelial Cells Partly via the Epithelial–Mesenchymal Transition of Epithelial Cells[J]. BioMed research international. 2018 Feb 8;2018:5015203.8.Zhenyu Wang, Weijian Li, Hongshu Jing, Ming Ding, Gong-Bo Fu, Tianjie Yuan, Weijian Huang, Mengjun Dai, Dan Tang, Min Zheng, Yi Chen, Hongdan Zhang, Yuan Peng, Qigen Li, Weifeng Yu, Hexin Yan, Bo Zhai . Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening. Theranostics.

引用文献

1.Wang Z, Li W, Jing H, et al. Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening. Theranostics. 2019, 9(22): 66902.Cai N, Xu B, Li X, et al. Roflumilast, a cyclic nucleotide phosphodiesterase 4 inhibitor, protects against cerebrovascular endothelial injury following cerebral ischemia/reperfusion by activating the Notch1/Hes1 pathway. European Journal of Pharmacology. 2022: 1750273.Huang C, Yan T, Xu Z, et al. Hypoxia Enhances Fusion of Oral Squamous Carcinoma Cells and Epithelial Cells Partly via the Epithelial–Mesenchymal Transition of Epithelial Cells. BioMed Research International. 2018 Feb 8;2018:50152034.Yang L, Wang X, Zhao G, et al.Leveraging temporal Wnt signal for efficient differentiation of intestinal stem cells in an organoid model.Stem Cells and Development.2023 (ja).5.Qu R, Peng Y, Zhou M, et al.MiR-223-3p attenuates M1 macrophage polarization via suppressing the Notch signaling pathway and NLRP3-mediated pyroptosis in experimental autoimmune uveitis.European Journal of Pharmacology.2023, 960: 176139.6.Zhan P, Lu Y, Lu J, et al.The activation of the Notch signaling pathway by UBE2C promotes the proliferation and metastasis of hepatocellular carcinoma.Scientific Reports.2024, 14(1): 22859.7.Xue J, Chu Y, Huang Y, et al.A tumorigenicity evaluation platform for cell therapies based on brain organoids.Translational Neurodegeneration.2024, 13(1): 53.8.Qin X, Fu L, Li C, et al.Optimized inner ear organoids for efficient hair cell generation and ototoxicity response modeling.Science China Life Sciences.2025: 1-15.

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