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IL-17 Protein, Canine, Recombinant

产品编号 TMPY-03906
IL-17 Protein, Canine, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15.2 kDa and the accession number is C6L8D7.
规格价格库存数量
100 μg
¥ 4,420
5日内发货
大包装 & 定制
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产品信息

生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized Canine IL17A at 10 μg/ml (100 μl/well) can bind human IL17Ra , The EC50 of human IL17Ra is 6.0-15.0 ng/ml. 2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED50 for this effect is typically 1-5 ng/mL.
产品描述
IL-17 Protein, Canine, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15.2 kDa and the accession number is C6L8D7.
种属
Canine
表达系统
E. coli
标签Tag Free
蛋白编号C6L8D7
别名
白细胞介素,白介素,interleukin 17A
蛋白构建
A DNA sequence encoding the canine IL17A (NP_001159350.1)(Gly26-Ala155) was expressed with a N-terminal Met. Predicted N terminal: Met
蛋白纯度
> 90 % as determined by SDS-PAGE
分子量15.2 kDa (predicted); 15 kDa (reducing condition, due to glycosylation)
内毒素Please contact us for more information.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

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