IL-17 Protein, Canine, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15.2 kDa and the accession number is C6L8D7.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 4,420 | 5日内发货 |
| 生物活性 | 1. Measured by its binding ability in a functional ELISA. Immobilized Canine IL17A at 10 μg/ml (100 μl/well) can bind human IL17Ra , The EC50 of human IL17Ra is 6.0-15.0 ng/ml.
2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED50 for this effect is typically 1-5 ng/mL. |
| 产品描述 | IL-17 Protein, Canine, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15.2 kDa and the accession number is C6L8D7. |
| 种属 | Canine |
| 表达系统 | E. coli |
| 标签 | Tag Free |
| 蛋白编号 | C6L8D7 |
| 别名 | 白细胞介素,白介素,interleukin 17A |
| 蛋白构建 | A DNA sequence encoding the canine IL17A (NP_001159350.1)(Gly26-Ala155) was expressed with a N-terminal Met. Predicted N terminal: Met |
| 蛋白纯度 | > 90 % as determined by SDS-PAGE |
| 分子量 | 15.2 kDa (predicted); 15 kDa (reducing condition, due to glycosylation) |
| 内毒素 | Please contact us for more information. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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