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IL-4 Protein, Mouse, Recombinant (CHO) is expressed in CHO Cells. The accession number is P07750.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 656 | 现货 | |
| 10 μg | ¥ 1,080 | 现货 | |
| 20 μg | ¥ 1,380 | 3日内发货 | |
| 50 μg | ¥ 1,980 | 现货 | |
| 100 μg | ¥ 3,230 | 5日内发货 | |
| 200 μg | ¥ 5,350 | 5日内发货 | |
| 500 μg | ¥ 9,870 | 5日内发货 | |
| 1 mg | ¥ 17,200 | 5日内发货 |
| 生物活性 | ED 50 < 2.0 ng/ml, measured in a cell proliferation assay using murine HT-2 cells, corresponding to a specific activity of > 5.0 × 10 5 units/mg. |
| 产品描述 | IL-4 Protein, Mouse, Recombinant (CHO) is expressed in CHO Cells. The accession number is P07750. |
| 种属 | Mouse |
| 表达系统 | CHO Cells |
| 标签 | Tag Free |
| 蛋白编号 | P07750 |
| 别名 | TCGF-2,T cell growth factor-2,MCGF-2,Mast-cell growth factor-2,IL4,BSF-1,BCGF-1,BCDF,B cell stimulatory factor-1,B cell growth factor-1,B cell differentiation factor |
| 蛋白构建 | His21-Ser140 |
| 蛋白纯度 | > 95% as determined by SDS-PAGE; > 95% as determined by HPLC |
| 分子量 | ~15 kDa (Reducing conditions) |
| 内毒素 | < 0.2 EU/μg of protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from a 0.2 μm filtered solution in PBS. |
| 复溶方法 | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 研究背景 | Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4 + T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4 + T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet. |