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GPRC5A Protein, Human, Recombinant (GST)

产品编号 TMPH-02032

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling. May act as a lung tumor suppressor. GPRC5A Protein, Human, Recombinant (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 37.0 kDa and the accession number is Q8NFJ5.

GPRC5A Protein, Human, Recombinant (GST)

GPRC5A Protein, Human, Recombinant (GST)

产品编号 TMPH-02032
Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling. May act as a lung tumor suppressor. GPRC5A Protein, Human, Recombinant (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 37.0 kDa and the accession number is Q8NFJ5.
规格价格库存数量
20 μg
¥ 1,800
20日内发货
100 μg
¥ 3,760
20日内发货
1 mg
¥ 16,000
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
细胞实验中,抑制剂如何溶解?
用 DMSO 配置较高浓度母液,母液存于 -80℃,建议多分装几管,一周内使用的存于 4℃,避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释,细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%,则需要在做预试验的时候,先进行溶剂阴性对照实验,排除溶剂对细胞的影响。
超声应使用什么样的频率?
频率建议使用常规的超声清洗频率即可,一般在20 kHz - 100 kHz。
超声久了会不会对化合物的结构有影响?
建议以较低频率对化合物进行超声,不会对化合物的结构造成影响。
超声应使用什么仪器?
建议使用“超声清洗仪”,不推荐用“超声破碎仪”。
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling. May act as a lung tumor suppressor. GPRC5A Protein, Human, Recombinant (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 37.0 kDa and the accession number is Q8NFJ5.
种属
Human
表达系统
E. coli
标签N-GST
蛋白编号Q8NFJ5
别名
Retinoic acid-induced protein 3,Retinoic acid-induced gene 1 protein (RAIG-1),RAIG1,RAI3,Phorbol ester induced gene 1 (PEIG-1),G-protein coupled receptor family C group 5 member A,GPRC5A,GPCR5A
氨基酸序列
TKQRNPMDYPVEDAFCKPQLVKKSYGVENRAYSQEEITQGFEETGDTLYAPYSTHFQLQNQPPQKEFSIPRAHAWPSPYKDYEVKKEGS
蛋白构建
269-357 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量37.0 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling. May act as a lung tumor suppressor.

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.

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